- African Art Museum
- Air and Space Museum and Udvar-Hazy Center
- American Art Museum and its Renwick Gallery
- American History Museum
- American Indian Museum
- Anacostia Museum (African American history and culture)
- Arts and Industries Building (Discovery Theater)
- Cooper-Hewitt National Design Museum
- Freer and Sackler Galleries (Asian art)
- Hirshhorn Museum and Sculpture Garden (modern and contemporary art)
- National Zoo
- Natural History Museum
- Portrait Gallery
- Postal Museum
- Smithsonian Institution Building (the Castle)